Nuclease Resistance - Introduction

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Introduction to Nuclease Resistance

Like cellular DNA and RNA, synthetic oligonucleotides are prone to degradation once introduced into the cell or body fluids. Such degradation is due to the ubiquitous presence of nuclease enzymes (both exonucleases and endonucleases), as well as chemical instability (particularly for RNA). Under normal cellular conditions, this leads to fast in vivo degradation of oligos and a short half-life (1). In addition, the ease with which RNAse, being very stable, can contaminate laboratory equipment and benchtop surfaces means that RNA is susceptible to degradation under normal laboratory conditions as well. To reduce or eliminate this susceptibility, nuclease-resistant modifications (for example, phosphorothiolation or 2'-OMethyl RNA bases) can be introduced into oligonucleotides slated for in vivo and/or regular benchtop work.

Nuclease Resistance Modifications						View PDF	Excel Mods & Code List
Product	Code	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	2 mmol scale (XX=03)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
2'-Fluoro deoxyadenosine (2'-F-A)	[fA]	26-6692	$42.00	$42.00	$50.00	$70.00	$265.00	$340.00
2'-Fluoro deoxycytosine (2'-F-C)	[fC]	26-6463	$27.00	$27.00	$39.00	$49.00	$214.00	$321.00
2'-Fluoro deoxyguanosine (2'-F-G)	[fG]	26-6693	$42.00	$42.00	$50.00	$70.00	$265.00	$340.00
2'-Fluoro deoxyuridine (2'-F-U)	[fU]	26-6462	$27.00	$27.00	$39.00	$49.00	$214.00	$321.00
2'-O methyl adenosine A	[mA]	27-6410A	$19.00	$19.00	$38.00	$57.00	$144.00	$175.00
2'-O methyl cytosine C	[mC]	27-6410C	$19.00	$19.00	$38.00	$57.00	$144.00	$175.00
2'-O methyl guanosine G	[mG]	27-6410G	$19.00	$19.00	$38.00	$57.00	$144.00	$175.00
2'-O methyl uridine U	[mU]	27-6410U	$19.00	$19.00	$38.00	$57.00	$144.00	$175.00
3'-dA (2'-5' linked)	[3dA2-5]	26-6490	$510.00	$510.00	$575.00	$748.00	$3,987.00	$4,984.00
3'-dC (2'-5' linked)	[3dC2-5]	26-6491	$590.00	$590.00	$645.00	$748.00	$3,987.00	$4,984.00
3'-dG (2'-5' linked)	[3dG2-5]	26-6492	$510.00	$510.00	$575.00	$748.00	$3,987.00	$4,984.00
3'-dT (2'-5' linked)	[3dT2-5]	26-6493	$510.00	$510.00	$575.00	$748.00	$3,987.00	$4,615.00
3'-O methyl bases (2'-5' linked)	[3OmN]	27-6458N	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rA (2'-5' linked)	[3OmA]	27-6458A	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rC (2'-5' linked)	[3OmC]	27-6458C	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rG (2'-5' linked)	[3OmG]	27-6458G	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rU (2'-5' linked)	[3OmU]	27-6458U	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-rA (2'-5' linked)	[3rA2-5]	26-6682	$410.00	$410.00	$575.00	$748.00	$3,987.00	$4,984.00
3'-rC (2'-5' linked)	[3rC2-5]	26-6911	$410.00	$410.00	$575.00	$748.00	$3,987.00	$4,984.00
3'-rG (2'-5' linked)	[3rG2-5]	26-6912	$410.00	$410.00	$575.00	$748.00	$3,987.00	$1,241.00
3'-rU (2'-5' linked)	[3rU2-5]	26-6913	$410.00	$410.00	$575.00	$748.00	$3,987.00	$1,241.00
L-DNA dA (beta L)	[L-dA]	26-6941	$119.00	$119.00	$178.00	$227.00	$781.00	$937.00
L-DNA dC (beta L)	[L-dC]	26-6942	$119.00	$119.00	$178.00	$227.00	$781.00	$937.00
L-DNA dG (beta L)	[L-dG]	26-6943	$119.00	$119.00	$178.00	$227.00	$781.00	$937.00
L-DNA dT (beta L)	[L-dT]	26-6944	$119.00	$119.00	$178.00	$227.00	$781.00	$937.00
L-RNA rA	[L-rA]	27-6941	$202.00	$202.00	$364.00	$545.00	$2,152.00	$2,447.00
L-RNA rC	[L-rC]	27-6942	$202.00	$202.00	$364.00	$545.00	$2,152.00	$2,447.00
L-RNA rG	[L-rG]	27-6943	$202.00	$202.00	$364.00	$545.00	$2,152.00	$2,447.00
L-RNA rU	[L-rU]	27-6944	$202.00	$202.00	$364.00	$545.00	$2,152.00	$2,447.00
Ethoxy Phosphate dA	[EoP-dA]	26-6641A	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Ethoxy Phosphate dC	[EoP-dC]	26-6641C	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Ethoxy Phosphate dG	[EoP-dG]	26-6641G	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Ethoxy Phosphate dT	[EoP-dT]	26-6641T	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Methoxy Phosphate dA	[MoP-dA]	26-6642A	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Methoxy Phosphate dC	[MoP-dC]	26-6642C	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Methoxy Phosphate dG	[MoP-dG]	26-6642G	$90.00	$90.00	$115.00	$145.00	$680.00	$880.00
Methoxy Phosphate dT	[MoP-dT]	26-6642T	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Phosphorothioate [Ps]	[Ps]	26-6401	$3.78	$4.59	$7.02	$9.72	$56.00	$70.00
propyne dC	[pdC]	26-6501	$140.00	$140.00	$216.00	$286.00	$1,728.00	$2,160.00
propyne dU	[pdU]	26-6502	$105.00	$105.00	$137.00	$205.00	$1,095.00	$1,369.00

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Nuclease Resistance - Introduction

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