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Conjugation & Surface Attachment - Introduction

Introduction to Conjugation & Surface Attachment

Conjugation & Surface Attachment Applications

Conjugation & Surface Attachment Design/Protocol

Conjugation & Surface Attachment Literature

Introduction to Conjugation & Surface Attachment

Incorporation of reactive organic functional groups, particularly primary amine, thiol (sulfhydryl), or carboxylate groups, at specific sites within an oligonucleotide allows for subsequent conjugation of the oligo with a number of different affinity, reporter or protein labels, depending on the application. Such labels need to be appropriately reactive to the incorporated functional group, for example, NHS esters or isothiocyanates in the case of primary amines, and iodoacetamides or maleimides in the case of primary thiols. Examples of labels includes fluorescent dyes/quenchers, digoxigenin, biotin, and enzymes. Functionally-derivitized oligos can also be covalently attached to surfaces such as glass slides or gold microspheres for use in various microarray or nanoelectronic applications.

The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.

Conjugation & Surface Attachment Modifications						View PDF	Excel Mods & Code List
Product	Code	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	2 mmol scale (XX=03)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
Alkyne-Modifier Serinol	[Alk-Ser]	26-6925	$260.00	$260.00	$338.00	$506.00	$2,701.00	$3,377.00
Alkyne-C2-(Propargyl-PEG1)	[Alk-C2-N]	26-6924	$200.00	$200.00	$297.00	$340.00	$1,011.00	$1,264.00
Amino Allyl	[AmAllyl]	26-6731	$227.00	$227.00	$276.00	$335.00	$1,674.00	$1,895.00
Aminoallyl rU	[AmAll-rU]	27-6548	$650.00	$650.00	$756.00	$824.00	$4,795.00	$5,551.00
Amino Mod C12-5'	[AmC12]	26-6420	$126.00	$126.00	$164.00	$246.00	$1,314.00	$1,643.00
Amino Mod C3-5	[AmC3-5]	26-6405F	$85.00	$85.00	$119.00	$142.00	$714.00	$856.00
Amino Mod C6	[AmC6]	26-6418	$85.00	$85.00	$119.00	$142.00	$714.00	$856.00
Amino C6 U (RNA)	[AmC6U]	27-6422	$875.00	$875.00	$910.00	$2,110.00	$4,421.00	$6,336.00
Amino deoxyadenosine dA C6	[Am-dA-C6]	26-6666	$267.00	$267.00	$298.00	$344.00	$1,717.00	$2,404.00
Amino dC N4 PEG3 amino linker	[Am-dC-C6]	26-6670	$267.00	$267.00	$298.00	$344.00	$1,717.00	$2,404.00
Amino deoxyguanosine dG C6	[Am-dG-C6]	26-6669	$518.00	$518.00	$551.00	$594.00	$2,538.00	$3,408.00
5'-Amino dT	[5-Am-dT]	26-6657T	$210.00	$210.00	$245.00	$286.00	$610.00	$785.00
Amino deoxythymidine dT C6	[Am-dT-C6]	26-6438	$199.00	$199.00	$228.00	$297.00	$1,485.00	$1,782.00
Amino modifier serinol	[Am-Ser]	26-6715	$289.00	$289.00	$347.00	$563.00	$3,002.00	$3,753.00
Amino Spacer 7 C6 Internal	[AmSp7-C6-Int]	26-6560	$227.00	$227.00	$406.00	$620.00	$2,484.00	$3,408.00
Amino Spacer 7-C6 (3')	[AmSp7-C6]	26-6425	$49.00	$49.00	$63.00	$95.00	$505.00	$632.00
Azide butyrate NHS	[N3-C4-N]	26-6922	$130.00	$130.00	$168.00	$253.00	$1,348.00	$1,685.00
5-Carboxy-C10	[CO-C10]	26-6717	$136.00	$136.00	$185.00	$210.00	$778.00	$1,011.00
Carboxy-dT	[CO-dT]	26-6697	$341.00	$341.00	$411.00	$524.00	$2,426.00	$3,033.00
Maleimide-Modifier (5')	[Mal]	26-6574	$113.00	$113.00	$147.00	$221.00	$881.00	$1,477.00
PC Amino C6 (Photocleavable)	[PCAmC6]	26-6690	$184.00	$184.00	$227.00	$356.00	$1,814.00	$2,268.00
6-Thio-dG (S6-dG)	[S6-dG]	26-6533	$551.00	$551.00	$684.00	$853.00	$4,609.00	$5,761.00
4-Thio dU (s4dU)	[s4dU]	26-6445	$413.00	$413.00	$537.00	$619.00	$4,294.00	$5,368.00
4-Thio-dT (S4dT)	[S4dT]	26-6538	$382.00	$382.00	$497.00	$573.00	$3,976.00	$4,970.00
4-Thio-Uridine (s4U)	[s4U]	27-6445	$536.00	$636.00	$676.00	$823.00	$3,616.00	$6,026.00
Thiol C6 dT	[S-C6-dT]	26-6483	$270.00	$270.00	$385.00	$494.00	$2,398.00	$3,996.00
Thiol SS Serinol Dipod (DTSPA)	[DTSPA]	26-6573	$310.00	$310.00	$403.00	$465.00	$3,224.00	$4,029.00
Thiol SS-C3	[SS-C3]	26-6482	$227.00	$227.00	$313.00	$427.00	$2,297.00	$2,646.00
Thiol SS-C6	[SS-C6]	26-6419	$106.00	$106.00	$138.00	$206.00	$1,101.00	$1,376.00

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Conjugation & Surface Attachment - Introduction

Introduction to Conjugation & Surface Attachment

Conjugation & Surface Attachment Modifications