propyne dU

Code : [pdU]

Modification : propyne dU

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6502
Antisense
[pdU]
Y
Y
Y
328.22
8.4
PS26-6502.pdf
-
-
-

Catalog No	Scale	Price
26-6502-05	50 nmol	$105.00
26-6502-02	200 nmol	$105.00
26-6502-01	1 umol	$137.00
26-6502-03	2 umol	$205.00
26-6502-06	5 umol	$616.50
26-6502-10	10 umol	$1,095.00
26-6502-15	15 umol	$1,369.00

Discounts are available for propyne dU!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

Related Modifications
2'-F-C	5-ca dC	N4-Ethyl dC [N4-Et-dC]
2'-O methyl G	2'-O methyl U	N6-Methyl dA (m6dA)
2'-F-A	2'-F-G	2'-O methyl A
2'-F-U	Thio-2-dT (S2dT)	5-Me dC
2'-O methyl C	2'-O methyl Inosine	AP-dC
2-Amino dA	propyne dC

It has been shown that C-5 propyne analogs of dC and dT when substituted in phosphorothioate oligonucleotide imparts greater inhibition of gene expression due to increased binding affinity to the target mRNA and increased stability. Based on the above information antisense oligonucleotide could either be Phosphorothioated at all diester linkages or combined with substitutions of dC and dT by C-5 propyne analogs pdC and pdU.

Modifications Increasing Duplex Stability and Nuclease Resistance
Modification	Duplex Stability [Tm Increase]	Nuclease Resistance
Locked Analog Bases	Increased [2- 4C per substitution]	Increased
2-Amino-dA	Increased [3.0C per substitution]	Similar to DNA
C-5 propynyl-C	Increased [2.8C per substitution]	Increased
C-5 propynyl-U	Increased [1.7C per substitution]	Increased
2'-Fluoro	Increased [1.8C per substitution]	Increased
5-Methyl-dC	Increased [1.3C per substitution]	Similar to DNA
2'-O Methyl	Increased	Increased
Phosphorothioate	Slightly decreased	Increased
Click here for complete list of duplex stability modifications

The use of propyne analogs is covered by patents and licensing agreements. The sale of propyne-modified oligos is for research use only. See license agreement* - propyne dU