5'-Amino dT

Amino dT 5'

Code : [5-Am-dT]

Modification : Amino dT 5'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6657T
Conjugation Chemistry
[5-Am-dT]
Y
N
N
303.21
8.4
26-6657T.pdf
260
-
8.4

Catalog No	Scale	Price
26-6657T-05	50 nmol	$210.00
26-6657T-02	200 nmol	$210.00
26-6657T-01	1 umol	$245.00
26-6657T-03	2 umol	$286.00
26-6657T-06	5 umol	$410.00
26-6657T-10	10 umol	$610.00
26-6657T-15	15 umol	$785.00

Discounts are available for Amino dT 5'!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

Related Modifications
Amino Serinol	Amino C6	Amino dT C6
Amino dG 5'	Amino dG C6	Amino dC 5'
Amino Spacer 7-C6 (3')	Amino C12-5'	Amino dA 5'

5'-Amino dT can be used to incorporate an active primary amino group into either a DNA oligonucleotide or a chimeric oligo at the 5'. This amino group is directly attached at the 5' end. The 5'-Amino dT modification does not have a spacer. The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).

The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.

- 5'-Amino dT