Spacer C12

Spacer modifications C3, C6, C9, C12 and triethylene glycol Spacer 9 and 18 are used to insert a spacer arm in an oligonucleotide. These modifications can be added in multiple additions when a longer spacer is required. 3'-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3'-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. See "Photo-Cleavable" modification category for photo-cleavable Spacer.

Spacer C12 is a 12-carbon spacer that is used to incorporate a long spacer arm into an oligonucleotide. Spacer C12 can be incorporated in consecutive additions if a longer spacer is required. Spacer C12 is frequently used for solid-phase immobilization of DNA probes or aptamers for microarray applications (1,2), but can be used for any oligonucleotide-based application requiring a long spacer arm.