Modification : Spacer 18
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
Catalog No | Scale | Price |
26-6447-05 | 50 nmol | $106.00 |
26-6447-02 | 200 nmol | $106.00 |
26-6447-01 | 1 umol | $159.00 |
26-6447-03 | 2 umol | $200.00 |
26-6447-06 | 5 umol | $715.50 |
26-6447-10 | 10 umol | $810.00 |
26-6447-15 | 15 umol | $945.00 |
Discounts are available for Spacer 18! |
Modification* Discount Price Structure |
1 site/order
|
List price
|
2 sites/order
|
10% discount
|
3 sites/order
|
20% discount
|
4 sites/order
|
30% discount
|
5-9 sites/order
|
50% discount
|
10+ sites/order
|
60% discount
|
*Exceptions apply
|
Spacer 18 is a hexaethylene glycol chain that is 18 atoms long (12 carbons + 6 oxygens), and is used to incorporate a long spacer arm into an oligonucleotide. Spacer 18 can be incorporated in consecutive additions whenever a longer spacer is required. Spacer 18 had been used to form bold folds and hairpin loops in oligonucleotides (1,2), and for solid-phase immobilization of hybridization probes (3). Spacer 18 has also been used to modify random primers used in whole genome amplification (WGA)-based applications, as a way to eliminate self-priming events that form spurious DNA products (that is, false-positive amplification) in the PCR reactions (4).
References
1. Salunkhe, M., Wu, T.F., Letsinger, R.L. Control of folding and binding of oligonucleotides by use of non-nucleotide linker.
J. Am. Chem. Soc. (1992),
114: 8768-8772.
2. Durand, M., Chevrie, K., Chassignol, M., Thuong, N.T., Maurizot, J. Circular dichroism studies of an oligodeoxyribonucleotide containing a hairpin loop made of a hexaethylene glycol chain: conformation and stability.
Nucleic Acids Res. (1990),
18: 6353-6359.
3. Zhang, Y., Coyne, M.Y., Will, S.G., Levenson, C.H., Kawasaki, E.S. Single-base mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides.
Nucleic Acids Res. (1991),
19: 3929-3933.
4. Brukner, I., Paquin, B., Belouchi, M., Labuda, D., Krajinovic, M. Self-priming arrest by modified random oligonucleotides facilitates the quality control of whole genome amplification.
Anal. Biochem. (2005),
339: 345-347.
- Spacer 18 (hexaethyleneglycol)