Gene Detection Systems

PCRProber^TM alkaline phosphatase labeled probe is for amplification and non-radioactive detection of a trinucleotide repeat region amplified PCR product. The PCRProber^TM Kit comprises of a primer pair for PCR amplification followed by gel blot and chemiluminescent detection using the alkaline phosphatase oligonucleotide probe. Quantity supplied is sufficient for 100 amplifications.

Gene Link's PCRProber^TM Kit is based on PCR amplification followed by Southern blot chemiluminescent detection using an alkaline phosphatase labeled oligonucleotide probe. This kit is a safe and sensitive alternate to radioactive-based detection methods. The amplified products are resolved on a sequencing polyacrylamide gel, and then blotted and processed for chemiluminescent detection.

The PCRProber^TM Kit is simple and robust for routine triple repeat detection of greater than 100 repeats of all triple repeat disorders listed, except Fragile X. The CGG repeat in Fragile X can be detected up to ~50 repeats.

It is strongly recommended that the genotyping be followed up by using GeneProber^TM Southern blot detection methods when two alleles are not clearly discernable.

Fragile X CGG repeats genotyping results using Gene Link's PCRProberTM. Various human genomic DNA samples were amplified using the reagents supplied in the PCRProberTM Kit (Catalog No. 40-2004-32) and processed for chemiluminescent detection following the protocol in the manual provided. Fragile X CGG repeat genotyping as an initial screening using the PCRProberTM is rapid as it amplifies CGG repeat sizes up to ~50 repeats. Lane 1 in the blot shows a female sample containing 29 and ~60 CGG repeats respectively.