Gene Detection Systems

Genemer^TM are optimized primer pairs for amplification of the gene fragment of interest, particularly those spanning a mutation. A wide range is available from single base pair mutation sites, the complex triple repeat disorders and various pathogens. Genemer^TM kits are complete, easy-to-use systems for reliable genotyping of large repeats in certain triple repeat disorder.

Gene Link has developed a wide array of specific amplification primers and optimized conditions for gene fragments and specifically for the triple repeat disorders. For example, the Huntington Genemer^TM kit is capable of routinely amplifying greater than 150 CAG repeats. Other detection systems available are for single base mutations and various pathogens.

The Genemer^TM product contains 10 nmols of the primer pair that is sufficient for 400 regular 50 µl amplifications. The Genemer^TM Kit contains reagents as well as control DNA sufficient for 100 amplifications.

Friedreich's ataxia GAA repeat genotyping was performed using FRDA GenemerTM (Catalog No. 40- 2027-10) and various FRDA GenemerTM Control DNAs (Catalog No. 40- 2027-XX). Optimized reagents from the FRDA GenemerTM Kit were used to amplify long repeats. Lanes 1 & 6 are molecular weight markers; lanes 2–5 are 64, 102, 110 and 125 GAA repeat amplification products.