Modification : N6-Methyl dA (m6dA)
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
Catalog No | Scale | Price |
26-6601-05 | 50 nmol | $345.00 |
26-6601-02 | 200 nmol | $345.00 |
26-6601-01 | 1 umol | $415.00 |
26-6601-03 | 2 umol | $535.00 |
26-6601-06 | 5 umol | $1,026.00 |
26-6601-10 | 10 umol | $1,825.00 |
26-6601-15 | 15 umol | $2,282.00 |
Discounts are available for N6-Methyl dA (m6dA)! |
Modification* Discount Price Structure |
1 site/order
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List price
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2 sites/order
|
10% discount
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3 sites/order
|
20% discount
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4 sites/order
|
30% discount
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5-9 sites/order
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50% discount
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10+ sites/order
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60% discount
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*Exceptions apply
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N6-Methyl-deoxyadenosine (N6-Me-dA) is a methylated nucleoside base that to date has only been found in bacterial and protist DNA (1). In these organisms, N6-Me-dA plays several roles, including post-replicative DNA mis-match repair, chromosome compaction and regulation of gene expression (2). Adenine methylation also is essential for either the viability or virulence of a number of pathological bacterial strains (3). Because of these properties, there is considerable interest in the bacterial enzyme N6-DNA methyltransferase (which methylates adenine) as a potential target for developing new anti-microbials (4), as well as the need to confirm whether or not this enzyme is present in mammals, including human (5). N6-Me-dA-modified oligonucleotides can serve as important research tools in such studies.
References
1. Hattman, S. DNA-[adenine] methylation in lower eukaryotes.
Biochemistry (Mosc) (2005),
70: 550-558.
2. Wion, D., Casadesus, J. N(6)-methyl-adenine: an epigenetic signal for DNA-protein interactions.
Nat. Rev. Microbiol. (2006),
4: 183-192.
3. Heithoff, D.M., Sinsheimer, R.L., Low, D.A., Mahan, M.J. An essential role for DNA adenine methylation in bacterial virulence.
Science (1999),
284: 967-970.
4. Mashoon, N., Carroll, M., Pruss, C., Eberhard, J., Ishikawa, S., Estabrook, R.A., Reich, N. Functional characterization of Escherichia coli DNA adenine methyltransferase, a novel target for antibiotics.
J. Biol. Chem. (2004),
279: 52075-52081.
5. Ratel, D., Ravanat, J-L., Charles, M-P., Platet, N., Breuillaud, L., Lunardi, J., Berger, F., Wion, D. Undetectable levels of N6-methyl adenine in mouse DNA. Cloning and analysis of PRED28, a gene coding for a putative mammalian DNA adenine methyltransferase.
FEBS Microbiol. Lett. (2006),
580: 3179-3184.
- N6-Methyl dA (m6dA)