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Modification : Thio-2-rU (s2U)
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
27-6537
RNA Oligo Synthesis
[s2U]
Y
Y
Y
322.22
8.4
PS27-6537.pdf
260
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| Catalog No | Scale | Price |
| 27-6537-05 | 50 nmol | $767.00 |
| 27-6537-02 | 200 nmol | $767.00 |
| 27-6537-01 | 1 umol | $913.00 |
| 27-6537-03 | 2 umol | $1,043.00 |
| 27-6537-06 | 5 umol | $4,108.50 |
| Discounts are available for Thio-2-rU (s2U)! |
| Modification* Discount Price Structure |
|
1 site/order
|
List price
|
|
2 sites/order
|
10% discount
|
|
3 sites/order
|
20% discount
|
|
4 sites/order
|
30% discount
|
|
5-9 sites/order
|
50% discount
|
|
10+ sites/order
|
60% discount
|
|
*Exceptions apply
|
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The effect of 2-thiouridine on sugar conformation and RNA duplex thermodynamics has
been well documented (1,2). The presence of the 2-sulfur modification stabilizes the 3’-endo
sugar conformation at the nucleoside and nucleotide level (1). Wobble base pair specificity
can also be improved by substituting 2-thiouridine for uridine. Testa and co-workers (2)
have shown the at S2U favors S2U-A pairing more than S2U-G pairing, and more than U
favors U-A relative to U-G. The sulfur modification improves specificity while retaining
other key uridine activities.
In addition, thiolated uridine has been shown to improve the rate and fidelity of both
nonenzymatic (3) and ribozyme (4) catalyzed nucleotide addition in RNA synthesis.
References
1. Kumar, R.K.; Davis, D.R. Nuc. Acid. Res. 1997, 25(6), 1272-1280.
2. Testa, S.M.; Disney, M.D.; Turner, D.H.; Kierzek, R. Biochem. 1999, 38, 16655-16662.
3. Heuberger, B.D.; Pal, A.; Del Frate, F.; Topkar, V.V.; Szostak, J.W. J. Am. Chem. Soc., 2015, 137 (7), pp 2769–2775.
4. Prywes, N.; Michaels, Y.S.; Pal, A.; Oh, S.S., Szostak, J.W. Chem. Commun. 2016. 52, 6529-6532.
5. a) Kumar, R.K.; Davis, D.R. J. Org. Chem.1995, 60, 7726-7727. b) Gait, M.J. Oligonucleotide Synthesis-A Practical Approach 1990, Oxford University Press, London.
- 2-Thio-Uridine (s2U)
Oligo Purification