Genetic Tools and Reagents

Purification of DNA from agarose gels and concentration of DNA by ethanol precipitation are routine protocols used in all molecular biology laboratories. The Omni-Clean^TM system provides optimized reagents for rapid extraction of DNA from agarose gels, and for routine concentration of DNA. The DNA is concentrated, purified and completely salt-free.

Application - Purification of DNA fragments excised from agarose gels

The Omni-Clean^TM DNA Purification System takes advantage of the principle that DNA binds to powdered flint glass in the presence of chaotropic salts. This technique provides a rapid and efficient method for the purification of high quality DNA from solutions or agarose gel slices, and is suitable for cloning, sequencing, isotope labeling, and a host of other procedures.

Lanes 2 and 3 are fragments excised from agarose gel and purified using the Omni-Clean^TM column based purification system.

Lane 1 is plasmid extracted using Omni-Pure^TM plasmid purification system. Lane 2 is the lower fragment gel purified using the Omni-Clean^TM gel glass bead based purification system.