Genetic Tools and Reagents

Omni-mRNA^TM Pooled Reference mRNA

APPLICATION: Microarray reference mRNA, RTPCR, cloning, cDNA amplification

Omni-mRNA^TM pooled reference mRNA is compatible with all commercially available labeling systems. Other applications of pooled reference mRNA include RNA ELISA, Quantigene, HPSA, and a number of other RNA amplification/detection systems. Omni-mRNA^TM pooled reference mRNA size distribution is from ~5 kb to 200 bp. These can also be used for cloning mRNA of interest by RTPCR. A 1.3 kb and a ~500 bp amplified cDNA fragment of p53 are shown in the figure.

Human and Mouse Omni-mRNA^TM Amplified Pooled Reference mRNA (Catalog No. 08- 0100-50 and 08-0200- 50) was used for synthesis of First Strand cDNA (Catalog No. 10-0100-05 and 10-0300-05) and used as a template for amplification of rare and abundant messages. PCR products generated from Human Omni-cDNA^TM (lanes 2-5) and Mouse Omni-cDNA^TM (lanes 6-9). Individual lanes correspond to the following products; lanes 2 & 6 = GAPDH (~500 bp); lanes 3 & 4 = granulocyte-macrophage colony stimulating factor (GMCSF) (~250 bp); lanes 4 & 7 = tumor necrosis factor alpha (TNF-a) (~325 bp); lanes 5 & 8 = Interleukin-1 receptor alpha (IL-1R a) (~300 bp). Lane 1 = molecular weight markers (200 bp-1500 bp).

Omni-mRNA^TM Amplified Pooled Reference mRNA
Product	Size	Catalog No.
Human Omni-mRNA^TM Amplified Pooled Reference mRNA	25 µg	08-0100-25
Mouse Omni-mRNA^TM 08-0200-25 25 µg 395.00 Amplified Pooled Reference mRNA	25 µg	08-0200-25
Rat Omni-mRNA^TM Amplified Pooled Reference mRNA	25 µg	10-0300-05
Guinea Pig Omni-mRNA^TM Amplified Pooled Reference mRNA	25 µg	08-2100-25
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