In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Quality Control | Purification | Duplex Stability Mods | Modifications | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
Coronavirus disease (COVID-19): Primers, Probes & Synthetic Positive Controls for 2019-nCoV
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Overview
On this page you will find information about:
- 1. Laboratory testing for coronavirus disease 2019 (COVID-19) in suspected human cases
- 2. WHO interim guidance for laboratory biosafety related to COVID-19 virus
- 3. Molecular assays to diagnose COVID-19 virus
- 4. WHO refence laboratories providing confirmatory testing for COVID-19
1. WHO interim guidance for laboratory testing
The purpose of this document is to provide interim guidance to laboratories and stakeholders involved in laboratory testing of COVID-19 virus.
Read the document (updated 2 March 2020)
2. WHO interim guidance for laboratory biosafety related to COVID-19 virus
The purpose of this document is to provide interim guidance on laboratory biosafety related to the COVID-19 virus to laboratories and stakeholders involved in COVID-19 virus laboratory work. This also includes the packaging and shipment requirements for sending specimens to WHO reference laboratories providing confirmatory testing for COVID-19.
Read the document (uploaded 12 February 2020)
3. Molecular assays to diagnose 2019-nCoV
Several assays that detect the 2019-nCoV have been and are currently under development, both in-house and commercially. Some assays may detect only the novel virus and some may also detect other strains (e.g. SARS-CoV) that are genetically similar.
Read the document (uploaded 12 February 2020)
In-house developed molecular assays
Some groups shared their protocols which can be accessed below (Summary table and link to the protocols). In some cases, the groups will be willing to send reagents or reagent mixes prepared in their laboratories, with or without associated fees. It is strongly recommended to contact the developer if you need further assistance. The list is not exhaustive and is being updated regularly.
Summary table of available protocols
Country |
Institute |
Gene Targets |
China |
Charité |
RdRP, E, N |
Hong Kong SAR |
HKU |
ORF1b-nsp14, N |
Japan |
National Institute of Infectious Diseases, Department of Virology III |
Pancorona and multiple targets, Spike protein |
Thailand |
National Institute of Health |
N |
US |
US CDC |
Three targets in N gene |
France |
Institut Pasteur, Paris |
Two targets in RdRP |
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