Literature (Product Manuals, Specifications, MSDS)

Catalog No.	Product	PDF Guide
R26-6400-OA	Oligo Analysis Parameters

Oligonucleotide Physical Parameters
The Custom Oligonucleotide Synthesis (COS) Report accompanying each custom oligonucleotide contains extensive thermodynamic and physical data for each oligo. This technical sheet explains each of the values and the method used for arriving at a particular physical data. The figures may differ for the same oligo sequence by using different software applications, the reason being the selection of particular details and constants for arriving at the calculated value. An example is the salt concentration for calculating Tm; Gene Link software uses 50 mM monovalent (Na+ and K+) salt concentration as a default, which reasonably approximates the total monovalent salt concentration commonly used in PCR reactions.