Ligation Calculator

PART 1 : Convert double strand DNA (ds DNA) to pmoles and ends
DNA Name
ds DNA size in base pairs (bp)
Quantity in micogram (ug)

PART 2 : Ligation Calculator Generally ligations are tested at 1:3, 1:1, 3:1 insert:vector molar ratios.
Insert size in base pairs (bp)
Vector size in base pairs (bp)
Vector amount in ng	10 50 100 Other Value: