RNAi ExplorerTM Product Line Price List |
Standard siRNA SynthesisThe siRNA duplexes provided are synthesized as a single strand oligo and annealed after complete deprotection and purification steps are completed. Gene Link utilizes the most reliable standard RNA synthesis chemistry. The RNA duplex is supplied lyophilized and ready to use. |
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Standard Custom siRNA Duplex Price List |
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Standard Purity* |
RPC Purified* |
PAGE Purified* |
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Size |
Price |
Catalog No. |
Price |
Catalog No. | Price |
Catalog No |
2 nmol |
$90.00 |
27-6436-20 |
$165.00 |
27-6437-20 |
$280.00 |
27-6438-20 |
5 nmol |
$110.00 |
27-6436-50 |
$185.00 |
27-6437-50 |
$380.00 |
27-6438-50 |
10 nmol |
$145.00 |
27-6436-10 |
$210.00 |
27-6437-10 |
$465.00 |
27-6438-10 |
20 nmol |
$180.00 |
27-6436-02 |
$294.00 |
27-6437-02 |
$570.00 |
27-6438-02 |
50 nmol |
$225.00 |
27-6436-05 |
$475.00 |
27-6437-05 |
$720.00 |
27-6438-05 |
100 nmol |
$420.00 |
27-6436-01 |
$685.00 |
27-6437-01 |
$1,026.00 |
27-6438-01 |
| *Please see table below for description of purity levels for standard purity, RPC and gel purified. | ||||||
SmartBaseTM siRNA Duplex Yield & Price List.Click for Yield & Price List |
| Standard Purity | RPC Purified** | Gel Purified |
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Purity is greater than 75% Reverse phase purification recommended. Gel purification recommended to achieve purity above 95%. |
Purity 85% to 95% depending on oligo sequence and structure. Yield and purity will be lower for sequences with high GC content **RPC is reverse phase purification using a cartridge; a substitue for HPLC. |
Purity 98% to ~100% depending on oligo sequence and structure. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield. |
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siRNA (duplexed)
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Catalog No. |
27-6401-06 |
27-6401-05 |
27-6401-02 |
27-6401-10 |
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Purification |
20 nmol scale* |
50 nmol scale* |
200 nmol scale* |
1 umol scale* |
| Crude | $195 | $295 | $420 | $650 |
| RPC | $225 | $325 | $450 | $740 |
| Gel | NA | $370 | $495 | $800 |
| *Please see table below for synthesis scale yields for crude, RPC and gel purified. | ||||
| Crude Desalted | RPC Purified** | Gel Purified | |
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Scale |
nmols |
nmols |
nmols |
| 20 nmol | ~5 | ~1 | NA |
| 50 nmol | ~10 | ~5 | ~0.5 |
| 200 nmol | ~30 | ~15 | ~5 |
| 1 umol | ~150 | ~75 | ~25 |
| 10 umol | ~1500 | ~600 | ~250 |
| 15 umol | ~2250 | ~900 | ~375 |
| Purity & Yield |
Purity is greater than 75% Reverse phase purification recommended. Gel purification recommended to achieve purity above 95%. |
Purity 85% to 95% depending on oligo sequence and structure. Yield and purity will be lower for sequences with high GC content **RPC is reverse phase purification using a cartridge; a substitue for HPLC. |
Purity 98% to ~100% depending on oligo sequence and structure. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield. |
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Control siRNA Duplexes*
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Product |
Size |
Catalog Number |
Price,$ |
| Negative Control, non-silencing unlabeled | 5 nmols | 27-6411-05 | $118.80 |
| Negative Control, non-silencing unlabeled | 20 nmols | 27-6411-20 | $270.00 |
| Negative Control, non-silencing Fl labeled | 2 nmols | 27-6411-02FL | $118.80 |
| Negative Control, non-silencing Fl labeled | 10 nmols | 27-6411-10FL | $378.00 |
| Negative Control, non-silencing Fl labeled | 20 nmols | 27-6411-20FL | $534.60 |
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*Please note catalog number change. Old Catalog Number was 27-6410-XXX |
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Positive Controls
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| Human Vimentin; NM_003380 | 5 nmols | 27-6412-05 | $118.80 |
| Human Vimentin; NM_003380 | 20 nmols | 27-6412-20 | $270.00 |
| Human Beta Actin; NM_001101 | 5 nmols | 27-6413-05 | $118.80 |
| Human Beta Actin; NM_001101 | 20 nmols | 27-6413-20 | $270.00 |
| Mouse Beta Tubulin; AF312873 | 5 nmols | 27-6414-05 | $118.80 |
| Mouse Beta Tubulin; AF312873 | 20 nmols | 27-6414-20 | $270.00 |
| Rat Chromogranin-A; NM_021655 | 5 nmols | 27-6415-05 | $118.80 |
| Rat Chromogranin-A; NM_021655 | 20 nmols | 27-6415-20 | $270.00 |
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Fluorescein Labeled Controls
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| Human Vimentin; NM_003380; FL labeled | 2 nmols | 27-6412-02FL | $118.80 |
| Human Vimentin; NM_003380; FL labeled | 10 nmols | 27-6412-10FL | $378.00 |
| Human Beta Actin; NM_001101; FL labeled | 2 nmols | 27-6413-02FL | $118.80 |
| Human Beta Actin; NM_001101; FL labeled | 10 nmols | 27-6413-10FL | $378.00 |
| Mouse Beta Tubulin; AF312873; FL labeled | 2 nmols | 27-6414-02FL | $118.80 |
| Mouse Beta Tubulin; AF312873; FL labeled | 10 nmols | 27-6414-10FL | $378.00 |
| Rat Chromogranin-A; NM_021655;FL labeled | 2 nmols | 27-6415-02FL | $118.80 |
| Rat Chromogranin-A; NM_021655;FL labeled | 10 nmols | 27-6415-10FL | $378.00 |
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DNA oligonucleotides
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Product |
200 nmol scale |
1 mmol scale |
| DNA Oligo Synthesis | $1.80/base | $3.25/base |
| Gel Purification | $75.00 | $150.00 |
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Guaranteed RNAi ExplorerTM Kit
Guaranteed RNAi Explorer Kit is only available for Human and Mouse Reference Sequence (RefSeq) Accession Numbers. |
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Product |
Size |
Catalog Number |
Price |
| 3 Target siRNA's & 1 negative control duplex. Standard purity, 2 nmol each | 2 nmol each | 27-6402-02 | $360.00 |
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Guaranteed RNAi ExplorerTM
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Product |
Size |
Catalog Number |
Price |
| Pre-designed 3 siRNA duplex standard purity, 2 nmol each | 2 nmol each | 27-6430-02 | $350 |
| Pre-designed 3 siRNA duplex standard purity, 5 nmol each | 5 nmol each | 27-6430-05 | $450 |
| Pre-designed 3 siRNA duplex standard purity, 10 nmol each | 10 nmol each | 27-6430-10 | $630 |
| Pre-designed 3 siRNA duplex standard purity, 20 nmol each | 20 nmol each | 27-6430-20 | $775 |
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Handling & Storage All siRNA are shipped lyophilized at room temperature. These are stable when kept lyophilized at room temperature. Follow established stringent RNase free handling conditions. The lyophilized siRNA duplex should be stored immediately at -20o C. The lyophilized siRNA is stable for ~6 months at -20o C. *RNAi and siRNA RNA interference (RNAi) is a specific and sequence dependent targeted gene silencing activity. RNAi acts by post transcriptional degradation of mRNA by small interfering RNAs (siRNA's) of the same sequence. The silencing approaches 100% and has to be empirically determined and optimized. Not every siRNA can effectively down regulate a gene. The process of RNA interference varies by individual siRNA while some do not exhibit any interference at all. |
