Linkers

Synthetic Oligonucleotides

Linkers are used for various cloning strategies to introduce restriction sites in the DNA after ligation. Linkers are short synthetic palindromic sequences which self anneal to form blunt ended double stranded fragments. Linkers are supplied as phosphorylated and non-phosphorylated forms. The 5’ terminus of non-phosphorylated linkers can be phosphorylated if desired using T4 polynucleotide kinase and ATP.

All Gene Link supplied linkers pass stringent quality control protocols of ligation and cleavage. The product is supplied as a lyophilized powder, after reconstitution store at -20oC. Oligo purity is greater than 98% as determined by denaturing polyacrylamide gel electrophoresis.

Catalog #
Linker
Sequence
Quantity
Price

26-3200-02

Bam HI non-phosphorylated (5’ OH)

5’ CGGGATCCCG

20 m g

$40.00

26-3200-03

Bam HI phosphorylated (5’ P)

5’pCGGGATCCCG

20 m g

$50.00

26-3200-04

Bgl II non-phosphorylated (5’ OH)

5’ GAAGATCTTC

20 m g

$40.00

26-3200-05

Bgl II phosphorylated (5’ P)

5’ pGAAGATCTTC

20 m g

$50.00

26-3200-06

Cla I non-phosphorylated (5’ OH)

5’ CCATCGATGG

20 m g

$40.00

26-3200-07

Cla I phosphorylated (5’ P)

5’ pCCATCGATGG

20 m g

$50.00

26-3200-08

Eco RI non-phosphorylated (5’ OH)

5’ CCGGAATTCCGG

20 m g

$40.00

26-3200-09

Eco RI phosphorylated (5’ P)

5’ pCCGGAATTCCGG

20 m g

$50.00

26-3200-10

Hind III non-phosphorylated (5’ OH)

5’ CCAAGCTTGG

20 m g

$40.00

26-3200-11

Hind III phosphorylated (5’ P)

5’ pCCAAGCTTGG

20 m g

$50.00

26-3200-12

Nco I non-phosphorylated (5’ OH)

5’ CATGCCATGGCATG

20 m g

$40.00

26-3200-13

Nco I phosphorylated (5’ P)

5’ pCATGCCATGGCATG

20 m g

$50.00

26-3200-14

Not I non-phosphorylated (5’ OH)

5’ AGCGGCCGCT

20 m g

$40.00

26-3200-15

Not I phosphorylated (5’ P)

5’ pAGCGGCCGCT

20 m g

$50.00

26-3200-16

Pst I non-phosphorylated (5’ OH)

5’ GCTGCAGC

20 m g

$40.00

26-3200-17

Pst I phosphorylated (5’ P)

5’ pGCTGCAGC

20 m g

$50.00

26-3200-18

Pvu I non-phosphorylated (5’ OH)

5’ ATCGATCGAT

20 m g

$40.00

26-3200-19

Pvu I phosphorylated (5’ P)

5’ pATCGATCGAT

20 m g

$50.00

26-3200-20

Sma I non-phosphorylated (5’ OH)

5’ TCCCCCGGGGGA

20 m g

$40.00

26-3200-21

Sma I phosphorylated (5’ P)

5’ pTCCCCCGGGGGA

20 m g

$50.00

26-3200-22

Xba I non-phosphorylated (5’ OH)

5’ CTAGTCTAGACTAG

20 m g

$40.00

26-3200-23

Xba I phosphorylated (5’ P)

5’ pCTAGTCTAGACTAG

20 m g

$50.00

26-3200-24

Xho I non-phosphorylated (5’ OH)

5’ CCGCTCGAGCGG

20 m g

$40.00

26-3200-25

Xho I phosphorylated (5’ P)

5’ pCCGCTCGAGCGG

20 m g

$50.00

26-3200-26

Nhe I non-phosphorylated (5’ OH)

GGCTAGCC

20 m g

$40.00

26-3200-27

Nhe I phosphorylated (5’ P)

pGGCTAGCC

20 m g

$50.00

26-3200-28

Apa I non-phosphorylated (5’ OH)

GGGGCCCC

20 m g

$40.00

26-3200-29

Apa I phosphorylated (5’ P)

pGGGGCCCC

20 m g

$50.00

26-3200-30

Eag I non-phosphorylated (5’ OH)

CCGGCCGG

20 m g

$40.00

26-3200-31

Eag I phosphorylated (5’ P)

pCCGGCCGG

20 m g

$50.00

26-3200-32

Pac I non-phosphorylated (5’ OH)

GTTAATTAAC

20 m g

$40.00

26-3200-33

Pac I phosphorylated (5’ P)

pGTTAATTAAC

20 m g

$50.00

26-3200-34

Kpn I non-phosphorylated (5’ OH)

GGGTACCC

20 m g

$40.00

26-3200-35

Kpn I phosphorylated (5’ P)

pGGGTACCC

20 m g

$50.00

26-3200-36

Mlu I non-phosphorylated (5’ OH)

CGACGCGTCG

20 m g

$40.00

26-3200-37

Mlu I phosphorylated (5’ P)

pCGACGCGTCG

20 m g

$50.00

26-3200-38

Spe I non-phosphorylated (5’ OH)

GGACTAGTCC

20 m g

$40.00

26-3200-39

Spe I phosphorylated (5’ P)

pGGACTAGTCC

20 m g

$50.00



Prices subject to change without notice
All Gene Link products are for research use only.

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