Gene Link provides custom first strand cDNA synthesis service for RT-PCR. Each lot is tested for amplification of b-actin cDNA. The first strand cDNA is prepared from tissue provided by the customer or Gene Link will locate the appropriate tissue source. The amount supplied is sufficient for at least 50 amplifications. First strand cDNA is useful for amplifying a particular cDNA using PCR. The PCR reaction must be optimized using varying amounts of the cDNA. This optimization is particularly important when the target mRNA species is of low abundance. The protocol included is for amplifying beta-actin as a control to validate the quality of the ‘first strand cDNA’ supplied. The PCR conditions to amplify the target cDNA will be based on the primers selected. It should be noted that specific sequence primers as well as degenerate sequence primers can be used successfully to amplify the target sequence. The first strand cDNA is prepared from freshly obtained tissue and appropriately frozen during transportation. RNA is extracted using a modified version of the widely used and published method (Chomczynski,P. and Sacchi, N. (1987) Anal. Biochem. 162:156-159).Oligo dT is used to prime the synthesis of the first strand using Moloney Murine leukemia Virus (MMLV) Reverse Transcriptase. The amount supplied is sufficient for at least 50 amplifications. Each lot is tested for amplification of b-actin cDNA

Please see catalog for in stock cDNA  

First strand cDNA is useful for amplifying a particular cDNA using PCR. beta-actin PCR mix is included with the product for amplifying b-actin as a control to validate the quality of the ‘first strand cDNA’ supplied. The PCR conditions to amplify the target cDNA will be based on the primers selected. It should be noted that specific sequence primers as well as degenerate sequence primers could be used successfully to amplify. Each lot is tested for amplification of b-actin cDNA.

The first strand cDNA is prepared from freshly obtained tissue and appropriately frozen during transportation. RNA is extracted using the widely used and published method (Chomczynski,P. and Sacchi, N. (1987) Anal. Biochem. 162:156-159). Oligo dT is used to prime the synthesis of the first strand using Moloney Murine leukemia Virus (MMLV) Reverse Transcriptase. The amount supplied is sufficient for at least 50 amplifications.