2'-O-propargyl/Alkyne A

2'-O-propargyl A

Code : [PPG-2-O-A]

Modification : 2'-O-propargyl A

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

27-6918A
Click Chemistry
[PPG-2-O-A]
Y
Y
Y
366.72
15.4
PS27-6918
260
-
15.4

Catalog No	Scale	Price
27-6918A-05	50 nmol	$310.00
27-6918A-02	200 nmol	$310.00
27-6918A-01	1 umol	$375.00
27-6918A-03	2 umol	$548.00
27-6918A-06	5 umol	$1,426.00
27-6918A-10	10 umol	$2,376.00
27-6918A-15	15 umol	$2,970.00

Discounts are available for 2'-O-propargyl A!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

Related Modifications
3'-O-propargyl A 2'-5' linked	3'-rG (2'-5' linked)	2'-O-propargyl C
DBCO-TEG (5')	Azide C3 3'	Azide PEG4 NHS
DBCO-dT	Alkyne- Serinol	2'-O-propargyl U
DBCO PEG4 NHS	2'-O-propargyl G	3'-O-propargyl C 2'-5' linked
3'-rA (2'-5' linked)	Azide dT (5')	Alkyne-C2 NHS
Azide C6 (5')

Click here for a complete list of Click Chemistry Oligo Modifications

Propargyl refers to triple/alkyne bond structure next to a saturated position with the following structure HC≡C−CH2−,. Placing a propargyl group at the 3' end in conjunction with an azide at the 5' position can be ligated using click chemistry.

Ligation of an oligo containing a 5'-azide with an oligo containing a 3'-propargyl group using Click Chemistry leads to a triazole linkage that has been shown to have in vivo biocompatibility. This technique has been used to synthesize DNA constructs up to 300 bases in length. When the resultant triazole linkage was placed in a PCR template, various polymerases were able to copy the sequence correctly. The linkage has also been shown to be compatible with transcription and rolling circle amplification, as well as gene expression in E. coli. In the RNA world, a hammerhead ribozyme containing the triazole linkage at the substrate cleavage site has been shown to retain its activity. A large variety of applications is envisaged for this biocompatible chemical ligation.

An azide can be introduced at the 5' end of an oligo using Iodo-dT (5'); catalog number. 26-6926.

- 2'-O-propargyl/Alkyne A