Modification : Fam Serinol
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
26-6503
Fluorescent Dyes
[Fam-Ser]
Y
Y
Y
582.45
20.9
PS26-6503
492
517
-
| Catalog No | Scale | Price |
| 26-6503-05 | 50 nmol | $346.00 |
| 26-6503-02 | 200 nmol | $351.00 |
| 26-6503-01 | 1 umol | $416.00 |
| 26-6503-03 | 2 umol | $632.00 |
| 26-6503-06 | 5 umol | $1,872.00 |
| 26-6503-10 | 10 umol | $3,326.00 |
| 26-6503-15 | 15 umol | $4,158.00 |
| Discounts are available for Fam Serinol ! |
| Modification* Discount Price Structure |
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1 site/order
|
List price
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2 sites/order
|
10% discount
|
|
3 sites/order
|
20% discount
|
|
4 sites/order
|
30% discount
|
|
5-9 sites/order
|
50% discount
|
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10+ sites/order
|
60% discount
|
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*Exceptions apply
|
Click here for a list of fluorophores.
Fam serinol is 6-carboxyfluorescein (6-FAM) Serinol. This can be used as a non-nucleosidic modification for internal labeling and will be inserted in the backbone of the oligo and can be extended.
6-carboxyfluorescein (6-FAM) is the most commonly used fluorescent dye for labeling oligonucleotides. 6-FAM is reactive, water-soluble, and has an absorbance maximum of 492 nm and an emission maximum of 517 nm. 6-FAM plays a particularly important role in real-time PCR applications, being used as a reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). For such probes, 6-FAM is most commonly paired with the dark quencher BHQ-1, as the two have excellent spectral overlap. 6-FAM-labeled primers have also been used for bacterial SNP genotyping by allele-specific real-time PCR (4).
6-FAM can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with 6-FAM at the 5'-end can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.
References
1. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
PCR Methods Appl. (1995),
4: 1-6.
2. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection.
Nucleic Acids Res. (2000),
28: 3752-3761.
3. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization.
Nat. Biotechnol. (1996),
14: 303-308.
4. Huygens, F., Inman-Bamber, J., Nimmo-G.R., Munckhof, W., Schooneveldt, J., Harrison, B., McMahon, J.A., Giffard, P.M. Staphylococcus aureus Genotyping Using Novel Real-Time PCR Formats.
J. Clin. Microbiol. (2006),
44: 3712-3718.
- Fam Serinol Internal
Oligo Purification