dihydro rUracil (5-6 DH rU)

dihydro rU (5-6 DH rU)

Code : [5-6-DHrU]

Modification : dihydro rU (5-6 DH rU)

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

27-6683
Minor Bases
[5-6-DHrU]
Y
Y
Y
308.19
8.4
PS27-6683.pdf
260
-
-

Catalog No	Scale	Price
27-6683-05	50 nmol	$335.00
27-6683-02	200 nmol	$335.00
27-6683-01	1 umol	$603.00
27-6683-03	2 umol	$723.00
27-6683-06	5 umol	$2,713.50
27-6683-10	10 umol	$3,616.00
27-6683-15	15 umol	$6,026.00

Discounts are available for dihydro rU (5-6 DH rU)!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

Related Modifications
dihydro dU (5-6 DHdU)	dihydro dC (5-6 DHC)	dihydro dT (5-6 DHT)

Dihydro dU (5,6-DHU) is primarily used in studies of irradiative DNA damage and associated repair mechanisms. In the cell, 5,6-DHU DNA lesions are formed by gamma irradiation of deoxycytosine under anoxic conditions, resulting in deamination followed by addition of hydrogen at C5 and C6 of the base. DHU is highly mutagenic, leading to C-to-T transitions at the mutation site (because DNA polymerase inserts A opposite the 5,6-DHU lesion) (1). Because DHU is recognized and removed by endonuclease III and other eukaryotic endo III homologs, DHU-modified oligos are used in model systems for studying DNA damage and repair mechanisms.

References
1. Liu, J., Doetsch, P.W. Escherichia coli RNA and DNA polymerase bypasss of dihydrouracil: mutagenic potential via transcription and replication. Nucleic Acids Res. (1998), 26: 1707-1712.
- dihydro rUracil (5-6 DH rU)