NTA Mono (Nitrilotriacetate) Oligo Conjugate

NTA Mono

Code : [NTA]

Modification : NTA Mono

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6444
Affinity Ligands
[NTA]
Y
Y
Y
489.38
-
PS26-6444.pdf
-
-
-

Catalog No	Scale	Price
26-6444-05	50 nmol	$478.00
26-6444-02	200 nmol	$478.00
26-6444-01	1 umol	$675.00
26-6444-03	2 umol	$1,012.00
26-6444-06	5 umol	$3,037.50
26-6444-10	10 umol	$4,752.00
26-6444-15	15 umol	$5,702.00

Related Modifications
Thiol C6 dT	NTA Tris	Thiol SS Dipod (DTSPA)
Thiol SS-C6	Thiol SS-C3	Thiol SS Dipod (DTPA)

YIELD
NTA modified oligos.

~ 2 nmol final yield for 50 nmol scale synthesis.
~ 6 nmol final yield for 200 nmol scale synthesis.
~ 20 nmol final yield for 1 umol scale synthesis.

NTA-Oligo modified with nitrilotriacetate (NTA)is a post synthesis conjugation to thiol. The thiol group can be placed at the 5' and 3' and for internal positions DTSPA can be used. The price listed is for NTA modification and does not include thiol modification, oligo synthesis and purification charges

NTA-Oligo modified with nitrilotriacetate (NTA), which has high affinity to a His-tag on recombinant protein via the complexation of Ni2+.
It is a novel method to prepare a DNA-protein conjugate using histidine-tag (His-tag) chemistry.

References

1. J. Shimada, T. Maruyama, T. Hosogi, J. Tominaga, N. Kamiya, M. Goto,. Conjugation of DNA with protein using His-tag chemistry and its application to the aptamer-based detection system, Biotechnol. Lett. 30 (2008) 2001�2006.
2.J. Shimada et al DNA�enzyme conjugate that can detect thrombin. Anal. Biochem. 414 (2011) 103�108.
- NTA Mono (Nitrilotriacetate) Oligo Conjugate