Atto Rho101

Code : [AttoRho101-N]

Modification : Atto Rho101

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6970
Fluorescent Dyes
[AttoRho101-N]
Y
Y
Y
892
-
PS26-6970.pdf
586
610
-

Catalog No	Scale	Price
26-6970-05	50 nmol	$702.00
26-6970-02	200 nmol	$702.00
26-6970-01	1 umol	$913.00
26-6970-03	2 umol	$2,754.00
26-6970-06	5 umol	$4,108.50
26-6970-10	10 umol	$7,301.00
26-6970-15	15 umol	$9,126.00

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

YIELD
NHS based modifications are post synthesis conjugation performed using a primary amino group. The yield is lower as compared to direct automated coupling of modifications that are available as amidites. Approximate yield for various scales are given below.

~2 nmol final yield for 50 nmol scale synthesis.
~5 nmol final yield for 200 nmol scale synthesis.
~16 nmol final yield for 1 umol scale synthesis

ATTO Rho101 is a derivative of the well-known dye Rhodamine 101. Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, and high thermal and photo-stability. The dye is moderately hydrophilic. ATTO Rho101 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. - Atto Rho101