Tide Fluor 5 NHS

Tide Fluor 5 N

Code : [TF5-N]

Modification : Tide Fluor 5 N

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6604
Others
[TF5-N]
Y
Y
Y
865
-
PS26-6604.pdf
-
-
-

Catalog No	Scale	Price
26-6604-05	50 nmol	$421.00
26-6604-02	200 nmol	$421.00
26-6604-01	1 umol	$548.00
26-6604-03	2 umol	$821.00
26-6604-06	5 umol	$2,466.00
26-6604-10	10 umol	$4,380.00
26-6604-15	15 umol	$5,476.00

Discounts are available for Tide Fluor 5 N!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

Related Modifications
Cy5-5'

Click here for a list of fluorophores.

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis	Yield, nmols
50 nmol	2 nmol
200 nmol	5 nmol
1 umol	16 nmol
2 umol	30 nmol
5 umol	75 nmol
10 umol	150 nmol
15 umol	225 nmol
* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.
* Click here for RNA Oligos scale of synthesis and yield.
NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

Tide Fluor 5 is a fluorescent dye used for labeling oligonucleotides. Tide Fluor 5 has an absorbance maximum of 649 nm and an emission maximum of 664 nm, with spectral properties very similar to those of Cy5. Tide Fluor 5 has very high photostability, and its fluorescence is extremely high and insensitive to pH changes from 3-11. Tide Fluor 5 can be substituted for Cy5 in any FRET-based oligonucleotide assay, for example, TaqMan or Molecular Beacons, or as the reporter dye for fluorescent-based hybridization probes.

- Tide Fluor 5 NHS