Tet Azide

Tet-Azide

Code : [Tet-N3]

Modification : Tet-Azide

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6724
Click Chemistry
[Tet-N3]
Y
Y
Y
596.2
-
PS26-6724.pdf
-
-
-

Catalog No	Scale	Price
26-6724-05	50 nmol	$486.00
26-6724-02	200 nmol	$486.00
26-6724-01	1 umol	$948.00
26-6724-03	2 umol	$948.00
26-6724-06	5 umol	$4,266.00
26-6724-10	10 umol	$5,054.00
26-6724-15	15 umol	$6,318.00

This modification is a post synthesis conjugation to an alkyne or DBCO modification at the appropriate site for click conjugation.

TET (Tetrachloro fluorescein)-Azide is a fluorescent dye containing a terminal azide group. TET has an absorbance maximum of 522 nm and an emission maximum of 538 nm. The presence of the azide allows the user to use �Click Chemistry� (a [3+2] cycloaddition reaction between alkynes and azides, using copper (I) iodide as a catalyst) to conjugate the TET-Azide to a terminal alkyne-modified oligo with extremely high regioselectivity and efficiency (1,2). Preparation of the alkyne-modified oligo can be achieved using the 5�-Hexynyl modifier (see its respective tech sheet for details).

References
1. Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
2. Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- Tet Azide