Antisense Oligonucleotides

Antisense Oligonucleotides - Ordering Information / Price List

Custom Antisense Oligo Synthesis
Product	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
Phosphorothioates	26-6401-XX	3.50	4.25	6.50	50.00	65.00
Propyne dC or propyne dU*	26-6408-XX	130.00	130.00	200.00	1600.00	2000.00
2'O methyl bases	27-6410-XX	6.00	10.00	16.00	200.00	250.00
2'-5' linked bases	26-6414-XX	275.00	275.00	300.00	2400.00	3000.00

Purification
Product	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
Gel Purification	26-6400-XX	75.00	75.00	150.00	1500.00	1500.00
Reverse Phase Cartridge	26-6400-XX	30.00	30.00	90.00	750.00	750.00

Scale of Synthesis & Yield

Oligo Scale of Synthesis and Typical Yield of Unmodified DNA Oligos*
	Crude Desalted			RPC Purified**			Gel Purified
	20 mer oligo Typical Yield			30 mer oligo Typical Yield			50 mer oligo Typical Yield
Scale	A₂₆₀ Units	nmols	mg	A₂₆₀ Units	nmols	mg	A₂₆₀ Units	nmols	mg
50 nmol	8-10	30+	0.2-0.3	4-5	12+	0.1-0.16	NR* [1-2]	NR* [2-4]	NR* [0.03-0.06]
200 nmol	20-25	80+	0.6-0.8	8-12	24+	0.26-0.4	4-6	8+	0.13-0.2
1 umol	100-120	400+	3-4	40-50	90+	1.3-1.6	20-25	40+	0.6-0.8
Purity & Yield	Purity is greater than 80% depending on oligo sequence and structure. Refer to coupling efficiency table for oligo length dependent purity and yield. No further purification required for PCR and sequencing applications. Gel purification recommended for oligos above 50 mer and all applications involving cloning and mutagenesis. **RPC is reverse phase purification using a cartridge; a substitue for HPLC.			Purity 85% to 95% depending on oligo sequence and structure Yield and Purity will be lower for sequences with high GC content Not recommended for oligos longer than 35 mer. **RPC is reverse phase purification using a cartridge; a substitue for HPLC.			Purity 98% to ~100% depending on oligo sequence and structure Yield will gradually decrease as length of oligo increases. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield. *NR Not Recommended
*a. Typical yield stated in the table is for unmodified random sequence oligos. Reduction in yield is observed with high GC content oligos and those forming strong secondary structures. b. Reduced yield is expected with modified oligos. The reduction percentage varies with modification type and number of sites. Typical reduction is 10%-20% per modified site. c. Yield of 33µg/A260 unit for DNA oligos is calculated for an ~equimolar base composition. Long stretches of a single base or homopolymers will have variable yields. Example for homopolymeric 20 mer: dA(20) = ~25ug/A260 Unit; dC(20) = ~40ug/A260 Unit; dG(20) = ~32ug/A260 Unit and dT(20) = ~37 ug/A260 Unit

*Propyne Analog Use Agreement
Our agreement with Glen Research who in turn has an agreement with Isis Pharmaceuticals, Inc. allows us to sell to you C-5 Propynes and G-clamps that are ultimately used for RESEARCH PURPOSES ONLY. In accordance with this agreement, we must inform you of the uses to which these products may be put, which are described below. This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. (�Isis�). Purchase of this product includes a limited license to use this product solely for internal research. This license specifically excludes (and you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreign counterpart), (c) resale (including sale of any products or services that incorporate this product) or (d) gene functionalization activities (including products or services that incorporate data derived from gene functionalization activities) if such activities have commercial application, any and all of which require a separate license from Isis. Neither this product nor any product created through its use may be used in human clinical trials. In the event you have a separate agreement with Isis regarding this product, which explicitly states that the foregoing is not applicable to you, your use of this product will be governed by the terms of such agreement. In no event does the limited license included with the purchase of this product expand or alter the scope of the license granted pursuant to such agreement.