This service is for plasmid clone and PCR product identification. Unidirectional sequencing is performed using the standard primers or a primer sequence specified by the investigator. There is no additional charge for standard primers (T7, T3, M13, SP6, SK, KS). For custom primers there will be a charge for oligo synthesis. Accuracy of 97% is typically achieved for this service with a read length of 300-500 bases. Sequence data files will be emailed. Hard copies of electropherograms and sequence files and electronic files on disk compatible with ABI sequence analysis software will be mailed upon request at an additional cost of $12.50.
Turn around time for this service is 48-72 hrs. A 24-hr. rush service is available for an additional charge of $20.00. Failed runs or runs with less than 97% accuracy are repeated at no extra charge. Duplicate failed runs are attributed to DNA and a minimum charge of $25.00 is applied.
