Fluorescent Molecular Probes

siRNA:RNA Interference

Oligo Design & Synthesis

RNA

First Strand cDNA

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RT-PCRmer^TM

OligoProber^TM

Genetic Tools & Reagents

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GeneAssays^TM

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Single Cell RNA Sequencing: MULTI-seq LMO

Literature (Product Manuals, Specifications, MSDS)

FRAXE/FMR2/AFF2 CCG Repeat Genotyping GeneProberTM AFF2-AJ31Dig1

Catalog No. Product PDF Guide 40-2054-41 FRAXE/FMR2/AFF2 CCG Repeat Genotyping GeneProberTM AFF2-AJ31Dig1 We have developed a probe to be used for Southern blot analysis that reliably detects the AFF2 CCG triple repeat amplification. The presence of pre-mutated and fully expanded alleles in either gender, were confirmed by Southern blot analysis, which also enabled exclusion of methylation or repeat number mosaics as well as PCR failure. The use of this probe has been recommended as suitable for genotyping of pre-mutations, full mutations, and mosaics specifically for individuals presented for FRAXA screening with negative results to determine FRAXE status.