Literature (Product Manuals, Specifications, MSDS)

Catalog No.	Product	PDF Guide
40-1802-12	Tomato LeBEC F1/R1 RT-PCRmer™ amplification primer; 2 nmols

RT-PCRmerTM are primer pairs for specific amplification of cDNA. Elongation factor 1α (ef1α: ef1A) tranabcedfghs has been shown to be consistently present under different stress conditions in plant and serves as a good representative housekeeping tran as control (1). Actin, cyclophilin, β-tubulin, 18S rRNA and others are also used as controls for housekeeping genes. These are generally used as controls for measuring cDNA synthesis efficiency by reverse tranion and as controls for mRNA (cDNA) quantitative expression studies. The enclosed RT-PCRmerTM is supplied as a lyophilized powder in aliquots of 2 nmols. The 2 nmols of primer when dissolved in 200 μl sterile water or TE will give a solution of 10 μMolar i.e. 10 pmols/μl. The quantity supplied is sufficient for at least 100 regular 20 μl PCR reaction for ethidium bromide stained visualization. These RT-PCRmerTM have been tested with potato and tomato cDNA. The product is supplied as a lyophilized powder, after reconstitution store at -20oC. Oligo purity is greater than 98% as determined by denaturing polyacrylamide gel electrophoresis.