Literature (Product Manuals, Specifications, MSDS)

Catalog No.	Product	PDF Guide
40-5116-10	TCEP 0.5M solution; 1 mL

TCEP (tris(2-carboxyethyl)phosphine) is a component in Gene Link Omni-Pure™ RNA purification kits (40-4090-XX and 40-4080-XX). It is used as reducing agent to break disulfide bonds in RNase A and thus ensure high yield of RNA extraction by inhibiting RNase A activity.
TCEP is also recommended for reducing thiol labeled siRNA and other oligonucleotides.
Usage
It is 100X concentrated for use at 10 uL/mL to reduce RNase disulfide bonds and use at 0.1M for thiol disulfide bonds in oligos to reduce to SH bonds.