Literature (Product Manuals, Specifications, MSDS)

Catalog No.	Product	PDF Guide
FISH-PS	SmartBase™ FISH Probes Design for Enhanced Performance

SmartBase™ FISH Probes Design for Enhanced Performance
Probes for fluorescent in situ hybridization (FISH) are derived from repetitive chromosomal regions to paint a chromosome or are directed towards unique gene(s) for identification. These probes are usually developed from clones (BAC and cosmids) and more recently synthesized chemically as oligonucleotides appropriately labeled with fluorescent dyes. The oligo probes can be synthesized as tiles or longer sequences up to 250mer in length by Gene Link. Gene Link presents various design options for synthesizing ‘Smart’ FISH probes. Smart FISH probes can be synthesized in a predetermined way to exhibit the features that is desired; for instance to increase nuclease resistance we can substitute standard bases with 2’ Fluoro bases. Standard DNA probes are like natural molecules that are prone to degradation under normal conditions, ubiquitous nucleases as well as chemical instability lead to fast degradation with a finite half life. The premise of this product guide is to discuss various modifications that are offered by Gene Link that may be used for design and synthesis of Smart FISH Probes. There are four common features that are desirable and in particular can be improved by using a combination of available nucleic acid modifications that modify the phosphodiester linkages, nucleic acid bases, the sugar moieties and addition of various other functional groups.